HLA antibody titre crossmatch by flow cytometry
- Description
- Flow cytometry analysing IgG binding to recipient T and B lymphocytes from peripheral blood
- Clinical details
- This test is used to gauge the amount of donor specific antibody present. It allows assessment of whether the antibody may be removed to allow transplantation. If antibody removal is feasible recipient serum samples pre and post a 3litre plasma-exchange procedure will allow assessment of the number of Pex required for removal to allow transplantation.
- Related condition
- Reference range
- > 2.3 - positive
- Units
- RMF
- Synonyms
- crossmatch incompatible HLAi
- Testing site
- Synnovis : Reference Services : Guy's Hospital
- Laboratory
- Clinical Transplantation
- Sample type and volume required
- donor - 20mls EDTA blood recipient - 5ml clotted blood
- Call in advance
- Call 020 7188 1540 to book in a crossmatch
- Special sample instructions
- samples should be bled on day of testing
- Storage and transport
- UN3373 packaging
- Turnaround time
- 2 days
- Time limit for extra tests
- none
- Contacts
Clinical Transplantation Laboratory at Guy’s Hospital
Phone: 020 7188 1540
Clinical Transplantation Laboratory
Guy’s Hospital
Borough Wing – 3rd Floor
Great Maze Pond
London SE1 9RTEmail: gstt.ctl@nhs.net