Deceased donor crossmatch (on-call)
- Description
- Flow cytometry analysing IgG binding to donor T and B lymphocytes from peripheral blood, lymph node or spleen.
- Clinical details
- In solid organ transplantation the presence of HLA donor specific antibodies is associated with an increased risk of graft loss and graft dysfunction. Flow cytometric (FCXM) crossmatch tests are used to identify HLA donor specific antibodies. A crossmatch test is performed in deceased kidney, kidney pancreas transplantation and pancreatic islet transplantation.
- Related condition
- Reference range
sensitised: >2.3 - positive unsensitised: >4.0 - positive
- Units
- RMF
- Synonyms
- DCD DBD crossmatch urgent donor spleen lymph node PBL
- Testing site
- Synnovis : Reference Services : Guy's Hospital
- Laboratory
- Clinical Transplantation
- Sample type and volume required
- 2cm piece of spleen, Lymph nodes or 30ml EDTA blood
- Call in advance
- On call staff must be contacted by the designated person responsible for transplantation to request this test prior to sending samples. On call staff must be contacted by a person from Richard Bright Ward as soon as the samples arrive
- Special sample instructions
- Sample must be sent to Richard Bright Ward at Guy's Hospital
- Storage and transport
- Temperature controlled
- Turnaround time
- 5 hours
- Time limit for extra tests
- none
- Contacts
Clinical Transplantation Department at Guy’s Hospital
Phone: 020 7188 1540
Clinical Transplantation Department
Guy’s Hospital
Borough Wing – 3rd Floor
Great Maze Pond
London SE1 9RT