Vitamin B1 (thiamine pyrophosphate)
- Description
- A Gerstel Multi Purpose Sampler coupled with liquid chromatography and electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) Due to the relocation of the Nutristasis service to the Blackfriars Hub site, this test is not currently accredited by UKAS to ISO15189
- Clinical details
- Vitamin B1 is classed as a water soluble vitamin. Thiamine diphosphate (TDP) is the most abundant physiological form and is currently considered to be the best available marker of thiamine status. Whole blood is the matrix of choice for assessment of B1 status. B1 can be found at high concentrations in whole-grain foods, pork, liver, nuts, and pulses. Vitamin B1 is a cofactor for transketolase, pyruvate, and 2 oxogluterate dehydrogenase complexes. These complexes are required for the oxidative degredation of sugars and the mitochondrial synthesis of adenosine triphosphate (ATP), the energy currency of cells. Vitamin B1 is also required for biosynthesis of acetylcholine and lipids necessary for a healthy nervous system. Vitamin B1 deficiency was classically seen in populations where polished rice was the staple diet resulting in the disease known as berberi. In individuals with high alcohol consumption B1 deficiency gives rise to Wernicke-Korsakoff syndrome, a disease that affects the brain. Symptoms of B1 deficiency include muscle weakness, neuropathy and cardiovascular abnormalities.
- Related condition
- Reference range
66.5-200.0
- Units
- nmol/L
- Synonyms
- Thiamine
- Testing site
- Synnovis : Reference Services : Blackfriars Hub
- Laboratory
- Nutristasis Unit
- Sample type and volume required
- The preferred sample is whole blood EDTA. However, lithium heparin sample can be also used. Sample volume = 200μL of EDTA whole blood
- Call in advance
- No
- Special sample instructions
Protect specimens from light and store frozen at -20 degrees.
- Storage and transport
- Samples can be transported by first class post and are received by CSR on the 5th Floor, North Wing at St Thomas' Hospital. Samples are suitable for storage and transportation at 2-8°C for at least 5 days. Samples are stable at room temperature for up to 3 days
- Turnaround time
- 10 days
- Patient description
- Vitamin B1 (thiamine diphosphate) is measured by HPLC with fluorsence detection
- Contacts
Nutristasis Unit at Blackfriars Hub
Phone: 0204 591 0055
Email: Nutristasis@synnovis.co.uk
Nutristasis
Floor 1, Synnovis Hub
Friars Bridge Court
41-43 Blackfriars Road
London
SE1 8NZ