Prothrombin fragment 1+2
- Description
- Concentration of prothrombin fragment 1+2 (PF1+2) is ascertained with a sandwich ELISA. PF1+2 in test plasma is captured by antibodies bound to microtitre plate wells, incubated, and unbound material then washed off. A solution of antibody to PF1+2 that is conjugated to an enzyme is added to 'ag' onto any captured PF1+2. Unbound conjugate is washed off and a substrate for the enzyme is added, the product of the enzyme-substrate reaction being coloured. Colour intensity is in direct proportion to the degree of conjugate-binding, itself proportional to the amount of PF1+2 capture and thus, concentration.
- Clinical details
- Assembly of the prothrombinase complex on the activated platelet surface occurs when FXa binds to surface phospholipid via Gla domain-calcium interactions, in association with its co-factor FVa. The substrate, prothrombin, is converted to the serine protease thrombin via cleavage in two locations, Arg271 and Arg320. The first cleavage at Arg271 produces fragment 1+2 and the intermediate prethrombin 2, the fragment 1+2 being released as an activation peptide. Prethrombin 2 is cleaved at Arg320 to yield active thrombin. Detection of elevated levels of prothrombin fragment 1+2 is indicative of increased thrombin generation and a hypercoagulable state."
- Related condition
- Reference range
69 - 229
- Units
- pmol/l
- Testing site
- Synnovis : Blood Sciences : St Thomas' Hospital
- Laboratory
- Diagnostic Haemostasis and Thrombosis
- Sample type and volume required
- External requests: Citrated platelet poor plasma 375µL x 1 aliquot Internal requests: please refer to EPR label
- Special sample instructions
The sample should be analysed or manipulated & stored in the laboratory within 4 hours of venepuncture. Please ensure sample tubes are filled exactly to the fill-line as underfilling creates a dilution error and leads to inaccurate results.
- Turnaround time
- 14 - 21 days
- Contacts
Diagnostic Haemostasis and Thrombosis Department
St Thomas’ Hospital
Phone: 020 7188 2797
St Thomas’ Hospital
North Wing – 4th and 5th Floors
Westminster Bridge Road
London SE1 7EHGuy’s Hospital
Phone: 020 7188 7188 ext. 53860
Guy’s Hospital
Southwark Wing – 4th Floor
Great Maze Pond
London SE1 9RT
Outside core hours, contact Duty Haemostasis Biomedical Scientist